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a cgrp human mce  (MedChemExpress)


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    Structured Review

    MedChemExpress a cgrp human mce
    A Cgrp Human Mce, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+cgrp+human+mce/%CE%B1-CGRP/pm39208804-206-147-149
    Average 94 stars, based on 14 article reviews
    a cgrp human mce - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies synaptophysin Abcam Cat# ab8049; RRID: AB_2198854 SOX2 Cell signaling Cat# 3579; RRID: AB_2195767 MAP2 Millipore Cat# MAB3418; RRID: AB_11212326 MAP2 Abcam Cat# ab183830; RRID: AB_2895301 Neurofilament Abcam Cat# ab7795; RRID: AB_306084 Neurofilament Millipore Cat# AB1989, RRID: AB_91202 GFAP Invitrogen Cat# 130300; RRID: AB_86543 S-100b Sigma-merck Cat# S2532; RRID: AB_477499 NKX2-2 Abcam Cat# ab191077; RRID: AB_2811076 NKX6-1 Abcam Cat# ab221549; RRID: AB_2754979 PAX7 Abcam Cat# ab92317; RRID: AB_10561454 LBX1 Invitrogen Cat# PA564884; RRID: AB_2662958 LMX1B Sigma-Aldrich Cat# HPA073716; RRID: AB_2686630 PAX2 Abcam Cat# ab150391; RRID: AB_3065222 LHX1/5 Developmental Studies Hybridoma Bank Cat# 4F2; RRID: AB_531784 TCF7L2 Cell Signaling Cat# 2569T; RRID: AB_2199816 TBR1 Abcam Cat# ab31940; RRID: AB_2200219 GABA Sigma-merck Cat# A0310; RRID: AB_476667 Gephyrin Santa Cruz Cat# sc-25311; RRID: AB_627670 PHOX2B Santa Cruz Cat# sc-376997; RRID: AB_2813765 SOX10 R&D Cat# MAB2864; RRID: AB_2195180 OLIG3 R&D Cat# MAB2456; RRID: AB_2157678 VGLUT2 Millipore Cat# MAB5504; RRID: AB_2187552 PSD95 Santa Cruz Cat# sc-32290; RRID: AB_628114 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-3xFLAG-WPRE OBiO https://www.obiosh.com/ pAAV-hSyn-EGFP-3XFLAG-WPRE retrograde tracer virus OBiO https://www.obiosh.com/ rAAV2/1-hSyn-Cre-WPRE-hGH polyA BrainVTA Cat# PT-0136 rAAV2/9-EF1a-DIO-EGFP-WPRE-hGH polyA BrainVTA Cat# PT-0795 rAAV2/1-hSyn-ChrimsonR-tdTomato-WPRE-hGH polyA BrainVTA Cat# PT-1432 Chemicals, peptides, and recombinant proteins mTeSR Plus Stem Cell Technologies Cat# 100-0276 DMEM-F12 Life Technologies Cat# C11330500BT Neurobasal Media Life Technologies Cat# 21103049 BrainPhys Neuronal Medium stemcell Cat# 05790 KNOCKOUT(TM) SR invitrogen Cat# 10828028 Accutase Stem Cell Technologies Cat# 07920 Amino acids, non-essential Life Technologies Cat# 11140050 Penicillin/Streptomycin Life Technologies Cat# 15140122 Glutamax Life Technologies Ca# 35050061 b-Mercaptoethanol Sigma-Aldrich Ca# M3148 N2 Life Technologies Cat# 17502048 B27(-VA) invitrogen Cat#12587010 (Continued on next page) Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER D- (+)-Glucose solution (45% H2O) Sigma-Aldrich Cat# G8769 B27 Life Technologies Cat# 17504044 Insulin Beyotime Cat# P3376 EGF novoprotein Cat#C029B Retinoic acid (RA) Sigma-Aldrich Cat# R2625 NGF novoprotein Cat# C060 Matrigel BD Cat# 354230 Y-27632 MCE Cat# HY-10583 LDN193189 MCE Cat# HY-12071A SB431542 Abcam Cat# ab120163 CHIR99021 Stem Cell Technologies Cat# 72054 XAV939 Sigma-Aldrich Cat# X3004 PD325901 Selleck Cat# S1036 Purmorphamine stemcell Cat# D204 BMP4 Abcam Cat# ab238298 SHH R&D Cat# 464-SH-200 BDNF novoprotein Cat# C076 Bucladesine sodium MCE Cat# HY-B0764 Ascorbic acid Sigma-Aldrich Cat# A92902 O.C.T compound Tissue-Tek Cat# 4583 Bovine serum albumin Sangong Cat# A500023-0100 Triton X-100 Sigma-Aldrich Cat# T8787 DAPI Solarbio Cat# C0065 Agarose, low gelling temperature Sigma-Aldrich Cat# A9414 ProLong Gold Antifade Reagent ThermoFisher Cat# P36930 Muscimol MCE Cat# HY-N2313 (R)-Baclofen MCE Cat# HY-17354 Bicuculline MCE Cat# HY-N0219 D-AP5 MCE Cat# HY-100714A CNQX disodium salt Tocris Cat# 1045 a-CGRP (human) MCE Cat# HY-P1071 Cal-520 AAT Bioquest Cat# 21130 powerload Invitrogen Cat# P10020 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme Cat# RC101 HiScript III All-in-one RT SuperMix Perfect Vazyme Cat# R333 Taq Pro Universal SYBR qPCR Master Mix Vazyme Cat# Q712 RapiClear Sunjin lab Cat# RC152001 Deposited data Raw and processed RNA-seq and scRNA-seq This paper GEO: GSE251680 RNA-seq for hESC and hCO and scRNA-seq for hCO Xiang et al.8 GEO: GSE97882 scRNA-seq for hThO Xiang et al.12 GEO: GSE122342 Reference transcriptome GRCh38 10X genomics https://support.10xgenomics.com/ Microarray dataset for human medulla Allen brain https://human.brain-map.org/ scRNA-seq for developing human brain Braun et al.23 https://github.com/linnarsson-lab/ developing-human-brain/ Experimental models: Cell lines H9 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ (Continued on next page) e2 Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER H1 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ hiPSCs RC01001A line Nuwacell https://www.nuwacell.com/ hiPSC RC01001B line Nuwacell https://www.nuwacell.com/ H9-AAVS1-CAG-GFP hESC line This paper N/A H9-AAVS1-CAG-mCherry hESC line This paper N/A Recombinant DNA and virus AAVS1-TALEN-L Gonzalez et al.36 Addgene # 59025 AAVS1-TALEN-R Gonzalez et al.36 Addgene # 59026 AAVS1-CAG-hrGFP Qian et al.37 Addgene # 52344 AAVS1-CAG-mCherry Chen et al.38 Addgene # 80946 Software and algorithms Maestro pro MEA system Axion Biosystems https://www.axionbiosystems.com/ Plexon Offline Sorter Plexon https://plexon.com/products/offline-sorter/ AxIS Navigator Axion Biosystems https://www.axionbiosystems.com/ Neural Metric Tool Axion Biosystems https://www.axionbiosystems.com/ Fiji N/A https://fiji.sc BioCAM DupleX 3Brain https://www.3brain.com/products/ single-well/biocam-duplex Brainwave5 3Brain https://www.3brain.com/products/ software/brainwave5 nf-core/rnaseq pipeline (v3.13.2) N/A https://github.com/nf-core/rnaseq STAR (v2.7.10a) N/A https://github.com/alexdobin/STAR RSEM (v1.3.1) N/A https://github.com/deweylab/RSEM GSVA (1.44.5) N/A https://github.com/rcastelo/GSVA Voxhunt (v 1.0.1) Fleck et al.24 https://github.com/quadbio/VoxHunt Cell Ranger (v6.1.2) N/A https://www.10xgenomics.com/support/ software/cell-ranger Seurat (v 4.3.0) Hao et al.39 https://satijalab.org/seurat/ DoubletFinder (v 2.0.3) McGinnis et al.40 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SeuratWrappers (v 0.3.1) N/A https://github.com/satijalab/seurat-wrappers AUCell (v 1.18.1) N/A https://github.com/aertslab/AUCell SCENIC (v 1.3.1) Sande et al.41 https://github.com/aertslab/SCENIC Harmony (v 0.1.1) Korsunsky et al.42 https://github.com/immunogenomics/harmony scvelo (v0.2.5) Bergen et al.43 https://github.com/theislab/scvelo Other Orbital shaker IKA KS260 Nucleofector Lonza AAB-1001 Micropipette Puller RWD MP-500 Glass microelectrode injection pump RWD R480 U-bottom ultra-low-attachment 96-well plate Corning CLS7007-24EA Tissue Culture Plate Insert 3.0um BIOFIL TCS002006 Ultra-low-attachment 6-well plate BIOFIL TCP030006 CytoView MEA plate (six-well) Axion Biosystems M384-tMEA-6W

    Real-time Polymerase Chain Reaction:

    Article Title: Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies synaptophysin Abcam Cat# ab8049; RRID: AB_2198854 SOX2 Cell signaling Cat# 3579; RRID: AB_2195767 MAP2 Millipore Cat# MAB3418; RRID: AB_11212326 MAP2 Abcam Cat# ab183830; RRID: AB_2895301 Neurofilament Abcam Cat# ab7795; RRID: AB_306084 Neurofilament Millipore Cat# AB1989, RRID: AB_91202 GFAP Invitrogen Cat# 130300; RRID: AB_86543 S-100b Sigma-merck Cat# S2532; RRID: AB_477499 NKX2-2 Abcam Cat# ab191077; RRID: AB_2811076 NKX6-1 Abcam Cat# ab221549; RRID: AB_2754979 PAX7 Abcam Cat# ab92317; RRID: AB_10561454 LBX1 Invitrogen Cat# PA564884; RRID: AB_2662958 LMX1B Sigma-Aldrich Cat# HPA073716; RRID: AB_2686630 PAX2 Abcam Cat# ab150391; RRID: AB_3065222 LHX1/5 Developmental Studies Hybridoma Bank Cat# 4F2; RRID: AB_531784 TCF7L2 Cell Signaling Cat# 2569T; RRID: AB_2199816 TBR1 Abcam Cat# ab31940; RRID: AB_2200219 GABA Sigma-merck Cat# A0310; RRID: AB_476667 Gephyrin Santa Cruz Cat# sc-25311; RRID: AB_627670 PHOX2B Santa Cruz Cat# sc-376997; RRID: AB_2813765 SOX10 R&D Cat# MAB2864; RRID: AB_2195180 OLIG3 R&D Cat# MAB2456; RRID: AB_2157678 VGLUT2 Millipore Cat# MAB5504; RRID: AB_2187552 PSD95 Santa Cruz Cat# sc-32290; RRID: AB_628114 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-3xFLAG-WPRE OBiO https://www.obiosh.com/ pAAV-hSyn-EGFP-3XFLAG-WPRE retrograde tracer virus OBiO https://www.obiosh.com/ rAAV2/1-hSyn-Cre-WPRE-hGH polyA BrainVTA Cat# PT-0136 rAAV2/9-EF1a-DIO-EGFP-WPRE-hGH polyA BrainVTA Cat# PT-0795 rAAV2/1-hSyn-ChrimsonR-tdTomato-WPRE-hGH polyA BrainVTA Cat# PT-1432 Chemicals, peptides, and recombinant proteins mTeSR Plus Stem Cell Technologies Cat# 100-0276 DMEM-F12 Life Technologies Cat# C11330500BT Neurobasal Media Life Technologies Cat# 21103049 BrainPhys Neuronal Medium stemcell Cat# 05790 KNOCKOUT(TM) SR invitrogen Cat# 10828028 Accutase Stem Cell Technologies Cat# 07920 Amino acids, non-essential Life Technologies Cat# 11140050 Penicillin/Streptomycin Life Technologies Cat# 15140122 Glutamax Life Technologies Ca# 35050061 b-Mercaptoethanol Sigma-Aldrich Ca# M3148 N2 Life Technologies Cat# 17502048 B27(-VA) invitrogen Cat#12587010 (Continued on next page) Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER D- (+)-Glucose solution (45% H2O) Sigma-Aldrich Cat# G8769 B27 Life Technologies Cat# 17504044 Insulin Beyotime Cat# P3376 EGF novoprotein Cat#C029B Retinoic acid (RA) Sigma-Aldrich Cat# R2625 NGF novoprotein Cat# C060 Matrigel BD Cat# 354230 Y-27632 MCE Cat# HY-10583 LDN193189 MCE Cat# HY-12071A SB431542 Abcam Cat# ab120163 CHIR99021 Stem Cell Technologies Cat# 72054 XAV939 Sigma-Aldrich Cat# X3004 PD325901 Selleck Cat# S1036 Purmorphamine stemcell Cat# D204 BMP4 Abcam Cat# ab238298 SHH R&D Cat# 464-SH-200 BDNF novoprotein Cat# C076 Bucladesine sodium MCE Cat# HY-B0764 Ascorbic acid Sigma-Aldrich Cat# A92902 O.C.T compound Tissue-Tek Cat# 4583 Bovine serum albumin Sangong Cat# A500023-0100 Triton X-100 Sigma-Aldrich Cat# T8787 DAPI Solarbio Cat# C0065 Agarose, low gelling temperature Sigma-Aldrich Cat# A9414 ProLong Gold Antifade Reagent ThermoFisher Cat# P36930 Muscimol MCE Cat# HY-N2313 (R)-Baclofen MCE Cat# HY-17354 Bicuculline MCE Cat# HY-N0219 D-AP5 MCE Cat# HY-100714A CNQX disodium salt Tocris Cat# 1045 a-CGRP (human) MCE Cat# HY-P1071 Cal-520 AAT Bioquest Cat# 21130 powerload Invitrogen Cat# P10020 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme Cat# RC101 HiScript III All-in-one RT SuperMix Perfect Vazyme Cat# R333 Taq Pro Universal SYBR qPCR Master Mix Vazyme Cat# Q712 RapiClear Sunjin lab Cat# RC152001 Deposited data Raw and processed RNA-seq and scRNA-seq This paper GEO: GSE251680 RNA-seq for hESC and hCO and scRNA-seq for hCO Xiang et al.8 GEO: GSE97882 scRNA-seq for hThO Xiang et al.12 GEO: GSE122342 Reference transcriptome GRCh38 10X genomics https://support.10xgenomics.com/ Microarray dataset for human medulla Allen brain https://human.brain-map.org/ scRNA-seq for developing human brain Braun et al.23 https://github.com/linnarsson-lab/ developing-human-brain/ Experimental models: Cell lines H9 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ (Continued on next page) e2 Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER H1 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ hiPSCs RC01001A line Nuwacell https://www.nuwacell.com/ hiPSC RC01001B line Nuwacell https://www.nuwacell.com/ H9-AAVS1-CAG-GFP hESC line This paper N/A H9-AAVS1-CAG-mCherry hESC line This paper N/A Recombinant DNA and virus AAVS1-TALEN-L Gonzalez et al.36 Addgene # 59025 AAVS1-TALEN-R Gonzalez et al.36 Addgene # 59026 AAVS1-CAG-hrGFP Qian et al.37 Addgene # 52344 AAVS1-CAG-mCherry Chen et al.38 Addgene # 80946 Software and algorithms Maestro pro MEA system Axion Biosystems https://www.axionbiosystems.com/ Plexon Offline Sorter Plexon https://plexon.com/products/offline-sorter/ AxIS Navigator Axion Biosystems https://www.axionbiosystems.com/ Neural Metric Tool Axion Biosystems https://www.axionbiosystems.com/ Fiji N/A https://fiji.sc BioCAM DupleX 3Brain https://www.3brain.com/products/ single-well/biocam-duplex Brainwave5 3Brain https://www.3brain.com/products/ software/brainwave5 nf-core/rnaseq pipeline (v3.13.2) N/A https://github.com/nf-core/rnaseq STAR (v2.7.10a) N/A https://github.com/alexdobin/STAR RSEM (v1.3.1) N/A https://github.com/deweylab/RSEM GSVA (1.44.5) N/A https://github.com/rcastelo/GSVA Voxhunt (v 1.0.1) Fleck et al.24 https://github.com/quadbio/VoxHunt Cell Ranger (v6.1.2) N/A https://www.10xgenomics.com/support/ software/cell-ranger Seurat (v 4.3.0) Hao et al.39 https://satijalab.org/seurat/ DoubletFinder (v 2.0.3) McGinnis et al.40 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SeuratWrappers (v 0.3.1) N/A https://github.com/satijalab/seurat-wrappers AUCell (v 1.18.1) N/A https://github.com/aertslab/AUCell SCENIC (v 1.3.1) Sande et al.41 https://github.com/aertslab/SCENIC Harmony (v 0.1.1) Korsunsky et al.42 https://github.com/immunogenomics/harmony scvelo (v0.2.5) Bergen et al.43 https://github.com/theislab/scvelo Other Orbital shaker IKA KS260 Nucleofector Lonza AAB-1001 Micropipette Puller RWD MP-500 Glass microelectrode injection pump RWD R480 U-bottom ultra-low-attachment 96-well plate Corning CLS7007-24EA Tissue Culture Plate Insert 3.0um BIOFIL TCS002006 Ultra-low-attachment 6-well plate BIOFIL TCP030006 CytoView MEA plate (six-well) Axion Biosystems M384-tMEA-6W

    RNA Sequencing:

    Article Title: Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies synaptophysin Abcam Cat# ab8049; RRID: AB_2198854 SOX2 Cell signaling Cat# 3579; RRID: AB_2195767 MAP2 Millipore Cat# MAB3418; RRID: AB_11212326 MAP2 Abcam Cat# ab183830; RRID: AB_2895301 Neurofilament Abcam Cat# ab7795; RRID: AB_306084 Neurofilament Millipore Cat# AB1989, RRID: AB_91202 GFAP Invitrogen Cat# 130300; RRID: AB_86543 S-100b Sigma-merck Cat# S2532; RRID: AB_477499 NKX2-2 Abcam Cat# ab191077; RRID: AB_2811076 NKX6-1 Abcam Cat# ab221549; RRID: AB_2754979 PAX7 Abcam Cat# ab92317; RRID: AB_10561454 LBX1 Invitrogen Cat# PA564884; RRID: AB_2662958 LMX1B Sigma-Aldrich Cat# HPA073716; RRID: AB_2686630 PAX2 Abcam Cat# ab150391; RRID: AB_3065222 LHX1/5 Developmental Studies Hybridoma Bank Cat# 4F2; RRID: AB_531784 TCF7L2 Cell Signaling Cat# 2569T; RRID: AB_2199816 TBR1 Abcam Cat# ab31940; RRID: AB_2200219 GABA Sigma-merck Cat# A0310; RRID: AB_476667 Gephyrin Santa Cruz Cat# sc-25311; RRID: AB_627670 PHOX2B Santa Cruz Cat# sc-376997; RRID: AB_2813765 SOX10 R&D Cat# MAB2864; RRID: AB_2195180 OLIG3 R&D Cat# MAB2456; RRID: AB_2157678 VGLUT2 Millipore Cat# MAB5504; RRID: AB_2187552 PSD95 Santa Cruz Cat# sc-32290; RRID: AB_628114 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-3xFLAG-WPRE OBiO https://www.obiosh.com/ pAAV-hSyn-EGFP-3XFLAG-WPRE retrograde tracer virus OBiO https://www.obiosh.com/ rAAV2/1-hSyn-Cre-WPRE-hGH polyA BrainVTA Cat# PT-0136 rAAV2/9-EF1a-DIO-EGFP-WPRE-hGH polyA BrainVTA Cat# PT-0795 rAAV2/1-hSyn-ChrimsonR-tdTomato-WPRE-hGH polyA BrainVTA Cat# PT-1432 Chemicals, peptides, and recombinant proteins mTeSR Plus Stem Cell Technologies Cat# 100-0276 DMEM-F12 Life Technologies Cat# C11330500BT Neurobasal Media Life Technologies Cat# 21103049 BrainPhys Neuronal Medium stemcell Cat# 05790 KNOCKOUT(TM) SR invitrogen Cat# 10828028 Accutase Stem Cell Technologies Cat# 07920 Amino acids, non-essential Life Technologies Cat# 11140050 Penicillin/Streptomycin Life Technologies Cat# 15140122 Glutamax Life Technologies Ca# 35050061 b-Mercaptoethanol Sigma-Aldrich Ca# M3148 N2 Life Technologies Cat# 17502048 B27(-VA) invitrogen Cat#12587010 (Continued on next page) Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER D- (+)-Glucose solution (45% H2O) Sigma-Aldrich Cat# G8769 B27 Life Technologies Cat# 17504044 Insulin Beyotime Cat# P3376 EGF novoprotein Cat#C029B Retinoic acid (RA) Sigma-Aldrich Cat# R2625 NGF novoprotein Cat# C060 Matrigel BD Cat# 354230 Y-27632 MCE Cat# HY-10583 LDN193189 MCE Cat# HY-12071A SB431542 Abcam Cat# ab120163 CHIR99021 Stem Cell Technologies Cat# 72054 XAV939 Sigma-Aldrich Cat# X3004 PD325901 Selleck Cat# S1036 Purmorphamine stemcell Cat# D204 BMP4 Abcam Cat# ab238298 SHH R&D Cat# 464-SH-200 BDNF novoprotein Cat# C076 Bucladesine sodium MCE Cat# HY-B0764 Ascorbic acid Sigma-Aldrich Cat# A92902 O.C.T compound Tissue-Tek Cat# 4583 Bovine serum albumin Sangong Cat# A500023-0100 Triton X-100 Sigma-Aldrich Cat# T8787 DAPI Solarbio Cat# C0065 Agarose, low gelling temperature Sigma-Aldrich Cat# A9414 ProLong Gold Antifade Reagent ThermoFisher Cat# P36930 Muscimol MCE Cat# HY-N2313 (R)-Baclofen MCE Cat# HY-17354 Bicuculline MCE Cat# HY-N0219 D-AP5 MCE Cat# HY-100714A CNQX disodium salt Tocris Cat# 1045 a-CGRP (human) MCE Cat# HY-P1071 Cal-520 AAT Bioquest Cat# 21130 powerload Invitrogen Cat# P10020 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme Cat# RC101 HiScript III All-in-one RT SuperMix Perfect Vazyme Cat# R333 Taq Pro Universal SYBR qPCR Master Mix Vazyme Cat# Q712 RapiClear Sunjin lab Cat# RC152001 Deposited data Raw and processed RNA-seq and scRNA-seq This paper GEO: GSE251680 RNA-seq for hESC and hCO and scRNA-seq for hCO Xiang et al.8 GEO: GSE97882 scRNA-seq for hThO Xiang et al.12 GEO: GSE122342 Reference transcriptome GRCh38 10X genomics https://support.10xgenomics.com/ Microarray dataset for human medulla Allen brain https://human.brain-map.org/ scRNA-seq for developing human brain Braun et al.23 https://github.com/linnarsson-lab/ developing-human-brain/ Experimental models: Cell lines H9 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ (Continued on next page) e2 Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER H1 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ hiPSCs RC01001A line Nuwacell https://www.nuwacell.com/ hiPSC RC01001B line Nuwacell https://www.nuwacell.com/ H9-AAVS1-CAG-GFP hESC line This paper N/A H9-AAVS1-CAG-mCherry hESC line This paper N/A Recombinant DNA and virus AAVS1-TALEN-L Gonzalez et al.36 Addgene # 59025 AAVS1-TALEN-R Gonzalez et al.36 Addgene # 59026 AAVS1-CAG-hrGFP Qian et al.37 Addgene # 52344 AAVS1-CAG-mCherry Chen et al.38 Addgene # 80946 Software and algorithms Maestro pro MEA system Axion Biosystems https://www.axionbiosystems.com/ Plexon Offline Sorter Plexon https://plexon.com/products/offline-sorter/ AxIS Navigator Axion Biosystems https://www.axionbiosystems.com/ Neural Metric Tool Axion Biosystems https://www.axionbiosystems.com/ Fiji N/A https://fiji.sc BioCAM DupleX 3Brain https://www.3brain.com/products/ single-well/biocam-duplex Brainwave5 3Brain https://www.3brain.com/products/ software/brainwave5 nf-core/rnaseq pipeline (v3.13.2) N/A https://github.com/nf-core/rnaseq STAR (v2.7.10a) N/A https://github.com/alexdobin/STAR RSEM (v1.3.1) N/A https://github.com/deweylab/RSEM GSVA (1.44.5) N/A https://github.com/rcastelo/GSVA Voxhunt (v 1.0.1) Fleck et al.24 https://github.com/quadbio/VoxHunt Cell Ranger (v6.1.2) N/A https://www.10xgenomics.com/support/ software/cell-ranger Seurat (v 4.3.0) Hao et al.39 https://satijalab.org/seurat/ DoubletFinder (v 2.0.3) McGinnis et al.40 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SeuratWrappers (v 0.3.1) N/A https://github.com/satijalab/seurat-wrappers AUCell (v 1.18.1) N/A https://github.com/aertslab/AUCell SCENIC (v 1.3.1) Sande et al.41 https://github.com/aertslab/SCENIC Harmony (v 0.1.1) Korsunsky et al.42 https://github.com/immunogenomics/harmony scvelo (v0.2.5) Bergen et al.43 https://github.com/theislab/scvelo Other Orbital shaker IKA KS260 Nucleofector Lonza AAB-1001 Micropipette Puller RWD MP-500 Glass microelectrode injection pump RWD R480 U-bottom ultra-low-attachment 96-well plate Corning CLS7007-24EA Tissue Culture Plate Insert 3.0um BIOFIL TCS002006 Ultra-low-attachment 6-well plate BIOFIL TCP030006 CytoView MEA plate (six-well) Axion Biosystems M384-tMEA-6W

    Microarray:

    Article Title: Generation of human region-specific brain organoids with medullary spinal trigeminal nuclei.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies synaptophysin Abcam Cat# ab8049; RRID: AB_2198854 SOX2 Cell signaling Cat# 3579; RRID: AB_2195767 MAP2 Millipore Cat# MAB3418; RRID: AB_11212326 MAP2 Abcam Cat# ab183830; RRID: AB_2895301 Neurofilament Abcam Cat# ab7795; RRID: AB_306084 Neurofilament Millipore Cat# AB1989, RRID: AB_91202 GFAP Invitrogen Cat# 130300; RRID: AB_86543 S-100b Sigma-merck Cat# S2532; RRID: AB_477499 NKX2-2 Abcam Cat# ab191077; RRID: AB_2811076 NKX6-1 Abcam Cat# ab221549; RRID: AB_2754979 PAX7 Abcam Cat# ab92317; RRID: AB_10561454 LBX1 Invitrogen Cat# PA564884; RRID: AB_2662958 LMX1B Sigma-Aldrich Cat# HPA073716; RRID: AB_2686630 PAX2 Abcam Cat# ab150391; RRID: AB_3065222 LHX1/5 Developmental Studies Hybridoma Bank Cat# 4F2; RRID: AB_531784 TCF7L2 Cell Signaling Cat# 2569T; RRID: AB_2199816 TBR1 Abcam Cat# ab31940; RRID: AB_2200219 GABA Sigma-merck Cat# A0310; RRID: AB_476667 Gephyrin Santa Cruz Cat# sc-25311; RRID: AB_627670 PHOX2B Santa Cruz Cat# sc-376997; RRID: AB_2813765 SOX10 R&D Cat# MAB2864; RRID: AB_2195180 OLIG3 R&D Cat# MAB2456; RRID: AB_2157678 VGLUT2 Millipore Cat# MAB5504; RRID: AB_2187552 PSD95 Santa Cruz Cat# sc-32290; RRID: AB_628114 Bacterial and virus strains pAAV-hSyn-EGFP-P2A-3xFLAG-WPRE OBiO https://www.obiosh.com/ pAAV-hSyn-EGFP-3XFLAG-WPRE retrograde tracer virus OBiO https://www.obiosh.com/ rAAV2/1-hSyn-Cre-WPRE-hGH polyA BrainVTA Cat# PT-0136 rAAV2/9-EF1a-DIO-EGFP-WPRE-hGH polyA BrainVTA Cat# PT-0795 rAAV2/1-hSyn-ChrimsonR-tdTomato-WPRE-hGH polyA BrainVTA Cat# PT-1432 Chemicals, peptides, and recombinant proteins mTeSR Plus Stem Cell Technologies Cat# 100-0276 DMEM-F12 Life Technologies Cat# C11330500BT Neurobasal Media Life Technologies Cat# 21103049 BrainPhys Neuronal Medium stemcell Cat# 05790 KNOCKOUT(TM) SR invitrogen Cat# 10828028 Accutase Stem Cell Technologies Cat# 07920 Amino acids, non-essential Life Technologies Cat# 11140050 Penicillin/Streptomycin Life Technologies Cat# 15140122 Glutamax Life Technologies Ca# 35050061 b-Mercaptoethanol Sigma-Aldrich Ca# M3148 N2 Life Technologies Cat# 17502048 B27(-VA) invitrogen Cat#12587010 (Continued on next page) Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER D- (+)-Glucose solution (45% H2O) Sigma-Aldrich Cat# G8769 B27 Life Technologies Cat# 17504044 Insulin Beyotime Cat# P3376 EGF novoprotein Cat#C029B Retinoic acid (RA) Sigma-Aldrich Cat# R2625 NGF novoprotein Cat# C060 Matrigel BD Cat# 354230 Y-27632 MCE Cat# HY-10583 LDN193189 MCE Cat# HY-12071A SB431542 Abcam Cat# ab120163 CHIR99021 Stem Cell Technologies Cat# 72054 XAV939 Sigma-Aldrich Cat# X3004 PD325901 Selleck Cat# S1036 Purmorphamine stemcell Cat# D204 BMP4 Abcam Cat# ab238298 SHH R&D Cat# 464-SH-200 BDNF novoprotein Cat# C076 Bucladesine sodium MCE Cat# HY-B0764 Ascorbic acid Sigma-Aldrich Cat# A92902 O.C.T compound Tissue-Tek Cat# 4583 Bovine serum albumin Sangong Cat# A500023-0100 Triton X-100 Sigma-Aldrich Cat# T8787 DAPI Solarbio Cat# C0065 Agarose, low gelling temperature Sigma-Aldrich Cat# A9414 ProLong Gold Antifade Reagent ThermoFisher Cat# P36930 Muscimol MCE Cat# HY-N2313 (R)-Baclofen MCE Cat# HY-17354 Bicuculline MCE Cat# HY-N0219 D-AP5 MCE Cat# HY-100714A CNQX disodium salt Tocris Cat# 1045 a-CGRP (human) MCE Cat# HY-P1071 Cal-520 AAT Bioquest Cat# 21130 powerload Invitrogen Cat# P10020 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme Cat# RC101 HiScript III All-in-one RT SuperMix Perfect Vazyme Cat# R333 Taq Pro Universal SYBR qPCR Master Mix Vazyme Cat# Q712 RapiClear Sunjin lab Cat# RC152001 Deposited data Raw and processed RNA-seq and scRNA-seq This paper GEO: GSE251680 RNA-seq for hESC and hCO and scRNA-seq for hCO Xiang et al.8 GEO: GSE97882 scRNA-seq for hThO Xiang et al.12 GEO: GSE122342 Reference transcriptome GRCh38 10X genomics https://support.10xgenomics.com/ Microarray dataset for human medulla Allen brain https://human.brain-map.org/ scRNA-seq for developing human brain Braun et al.23 https://github.com/linnarsson-lab/ developing-human-brain/ Experimental models: Cell lines H9 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ (Continued on next page) e2 Cell Stem Cell 31, 1–12.e1–e8, October 3, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER H1 hESC line WiCell https://www.ncbi.nlm.nih.gov/ pubmed/9804556/ hiPSCs RC01001A line Nuwacell https://www.nuwacell.com/ hiPSC RC01001B line Nuwacell https://www.nuwacell.com/ H9-AAVS1-CAG-GFP hESC line This paper N/A H9-AAVS1-CAG-mCherry hESC line This paper N/A Recombinant DNA and virus AAVS1-TALEN-L Gonzalez et al.36 Addgene # 59025 AAVS1-TALEN-R Gonzalez et al.36 Addgene # 59026 AAVS1-CAG-hrGFP Qian et al.37 Addgene # 52344 AAVS1-CAG-mCherry Chen et al.38 Addgene # 80946 Software and algorithms Maestro pro MEA system Axion Biosystems https://www.axionbiosystems.com/ Plexon Offline Sorter Plexon https://plexon.com/products/offline-sorter/ AxIS Navigator Axion Biosystems https://www.axionbiosystems.com/ Neural Metric Tool Axion Biosystems https://www.axionbiosystems.com/ Fiji N/A https://fiji.sc BioCAM DupleX 3Brain https://www.3brain.com/products/ single-well/biocam-duplex Brainwave5 3Brain https://www.3brain.com/products/ software/brainwave5 nf-core/rnaseq pipeline (v3.13.2) N/A https://github.com/nf-core/rnaseq STAR (v2.7.10a) N/A https://github.com/alexdobin/STAR RSEM (v1.3.1) N/A https://github.com/deweylab/RSEM GSVA (1.44.5) N/A https://github.com/rcastelo/GSVA Voxhunt (v 1.0.1) Fleck et al.24 https://github.com/quadbio/VoxHunt Cell Ranger (v6.1.2) N/A https://www.10xgenomics.com/support/ software/cell-ranger Seurat (v 4.3.0) Hao et al.39 https://satijalab.org/seurat/ DoubletFinder (v 2.0.3) McGinnis et al.40 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SeuratWrappers (v 0.3.1) N/A https://github.com/satijalab/seurat-wrappers AUCell (v 1.18.1) N/A https://github.com/aertslab/AUCell SCENIC (v 1.3.1) Sande et al.41 https://github.com/aertslab/SCENIC Harmony (v 0.1.1) Korsunsky et al.42 https://github.com/immunogenomics/harmony scvelo (v0.2.5) Bergen et al.43 https://github.com/theislab/scvelo Other Orbital shaker IKA KS260 Nucleofector Lonza AAB-1001 Micropipette Puller RWD MP-500 Glass microelectrode injection pump RWD R480 U-bottom ultra-low-attachment 96-well plate Corning CLS7007-24EA Tissue Culture Plate Insert 3.0um BIOFIL TCS002006 Ultra-low-attachment 6-well plate BIOFIL TCP030006 CytoView MEA plate (six-well) Axion Biosystems M384-tMEA-6W



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